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| Registros recuperados : 104 | |
| 11. |  | OLIVEIRA, J. A. da S.; QUINTAO, C. C. R.; ASCARI, I. J.; CAMARGO, L. S. de A. Efeito da técnica de biópsia no desenvolvimento de embriões bovinos produzidos in vitro. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | QUINTAO, C. C. R.; OLIVEIRA, C. S.; PEREIRA, M. M.; CAMARGO, L. S. de A.; SARAIVA, N. Z. Effects of forskolin supplementation at different stages of IVP on the preimplantation development of bovine embryos. Animal Reproduction, v. 19, n. 2, e22149, 2022. Edição dos abstracts da Annual Meeting of the Brazilian Embryo Technology Society, 35., Foz do Iguaçu, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | QUINTAO, C. C. R.; SARAIVA, N. Z.; OLIVEIRA, C. S.; CAMARGO, L. S. de A.; MUNK, M. Zinc oxide nanoparticles supplementation on in vitro maturation of bovine oocytes and its effects on the embryo quality, lipid accumulation, and preimplantation development. Animal Reproduction, v. 20, n. 2, 2023. Abstracts of 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | PEREIRA, M. M.; SERAPIAO, R. V.; QUINTAO, C. C. R.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. de A. Apoptosis in bovine embryos produced with oocytes matured in serum-free medium under low oxygen tension. Acta Scientiae Veterinariae, v. 38, p. s675, 2010. Supl.2.
Edição dos resumos da 25a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. Domestic Animal Endocrinology, v. 60, p. 50-60, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | CAMARGO, L. S. de A.; PALUDO, F.; PEREIRA, M. M.; WOHLRES-VIANA, S.; GIOSO, M. M.; CARVALHO, B. C. de; QUINTAO, C. C. R.; VIANA, J. H. M. Absence of Sperm Factors as in the Parthenogenesis Does Not Interfere on BovineEmbryo Sensitiveness to Heat Shock at Pre-Implantation Stage. Reproduction in Domestic Animals, v. 51, p. 3-9, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | QUINTÃO, C. C. R.; SERAPIÃO, R. V.; SINGULANI, J. DE L.; RAPOSO, N. R. B.; CAMARGO, L. S. de A.; VIANA, J. H. M. Citotoxicidade de Lippia Lacunosa e Lippia Rotundifolia em fibroblastos bovinos por diferentes métodos. In: REUNIÃO ANUAL DA FEDERAÇÃO DE SOCIEDADE DE BIOLOGIA EXPERIMENTAL, 24., 2009, Águas de Lindóia. Anais... Águas de Lindóia: FESBE, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | CAMARGO, L. S. de A.; GONÇALVES, F. S.; QUINTAO, C. C. R.; IGUMA, L. T.; SOUZA, E. D.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Genetic modification of bovine embryos by lentiviral vectors. Animal Reproduction, v.10, n.3, p. 359, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 104 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
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Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
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DOI: |
10.1016/j.domaniend.2017.03.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
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Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
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Thesaurus NAL: |
DNA fragmentation; heat stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
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001 2085044
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.domaniend.2017.03.003$2DOI
100 1 $aASCARI, I. J.
245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource]
260 $c2017
520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.
650 $aDNA fragmentation
650 $aheat stress
653 $aMitochondrial membrane potential
653 $aROS production
653 $aThermoprotection
700 1 $aALVES, N. G.
700 1 $aJASMIN, J.
700 1 $aLIMA, R. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aOBERLENDER, G.
700 1 $aMORAES, E. A.
700 1 $aCAMARGO, L. S. de A.
773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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